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KMID : 0385520080210040338
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Analytical Science & Technology
2008 Volume.21 No. 4 p.338 ~ p.343
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Evaluation of two DNA extraction methods on exhumed bone samples
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Kim Soon-Hee
Hong Seung-Bum
Brian M.
Kemp
Park Ki-Won
Han Myun-Soo
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Abstract
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Extraction of DNA from skeletal material is of great importance in the identification of human remains, but is particularly difficult because the high amount of microbial DNA was often co-extracted with human bone DNA. We found that a phenol/chloroform extraction, followed by ultrafiltration, and cleanup by via the QIAquick® PCR purification kit yields higher amounts of human genomic DNA compared with extraction by the column affinity method® alone. Ultrafiltration extraction of human DNA from ten exhumed bone samples yielded 0.041?1.120 ng/ μL DNA (mean = 0.498 ng/μL DNA), and purification using the column affinity resulted in 0.016?0.064 ng/μL DNA (mean = 0.034 ng/μL DNA). Although the STR genotyping by the column affinity method was partially successful, all DNA samples by the ultrafiltration method produced full profiles from the multiplex PCR. The efficiency of STR genotyping was in accordance with the amounts of the human DNA extracted.
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KEYWORD
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forensic sciences, DNA extraction, bone, microbial DNA contamination
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